5MMZ
Structure of PRL-1 in complex with the Bateman domain of CNNM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9723 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.730, 128.478, 153.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 98.600 - 2.400 |
| R-factor | 0.2266 |
| Rwork | 0.225 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iy0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.630 | 2.410 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 1.150 | |
| Number of reflections | 20944 | |
| <I/σ(I)> | 26.5 | 2.5 |
| Completeness [%] | 99.8 | 86 |
| Redundancy | 13 | 13.5 |
| CC(1/2) | 0.990 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2M Sodium Formate, 0.1M Sodium Acetate pH 4.6 |
