5MMH
The X-Ray Structure of the Effector Domain of the Transcriptional Regulator AmpR of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 106.725, 71.000, 124.010 |
| Unit cell angles | 90.00, 112.62, 90.00 |
Refinement procedure
| Resolution | 35.377 - 2.200 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.500 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 43111 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 98.9 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | : 100 mM Tris pH 8.5, supplemented with 8% PEG 4000 and 800 mM LiCl |
