5MM3
Unstructured MamC magnetite-binding protein located between two helices.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 115.285, 115.285, 117.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.180 - 2.100 |
| R-factor | 0.18445 |
| Rwork | 0.182 |
| R-free | 0.22781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e7u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.418 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.180 | |
| High resolution limit [Å] | 2.100 | |
| Rmeas | 0.118 | 0.430 |
| Number of reflections | 38573 | |
| <I/σ(I)> | 23.3 | 4.56 |
| Completeness [%] | 82.6 | 87.5 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.4 M tri-Sodium citrate and 0.1 M HEPES |
