5MLK
Biotin dependent carboxylase AccA3 dimer from Mycobacterium tuberculosis (Rv3285)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.974710 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.897, 85.206, 148.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.412 - 1.939 |
| R-factor | 0.1901 |
| Rwork | 0.189 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.079 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.939 | 1.940 |
| Rmeas | 0.123 | 0.973 |
| Number of reflections | 73716 | |
| <I/σ(I)> | 19.04 | 2.89 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 12.98 | 12.98 |
| CC(1/2) | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 25% PEG 3350, 0.1M Bis-Tris pH 6.5 |
