5MLG
Crystal structure of rat prorenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 |
| Unit cell lengths | 142.543, 142.543, 77.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.210 - 2.600 |
| R-factor | 0.2185 |
| Rwork | 0.218 |
| R-free | 0.23048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4amt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.400 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 | 0.929 |
| Number of reflections | 23787 | |
| <I/σ(I)> | 14.3 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.999 | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.9M K/NaH2PO4, 0.1M HEPES PH7.3 |
