5MLF
Structure of Psb29 at 1.55A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 138.910, 138.910, 305.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.977 - 3.637 |
| R-factor | 0.3134 |
| Rwork | 0.311 |
| R-free | 0.35750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mjr |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.471 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.980 | 3.730 |
| High resolution limit [Å] | 3.637 | 3.637 |
| Rmerge | 0.394 | 0.760 |
| Number of reflections | 20447 | |
| <I/σ(I)> | 1.8947 | 1.01 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.64 | 14.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 16% w/v PEG 6K, 80mM Na citrate pH 5 |
