5MKW
Crystal structure of the human ZRANB3 HNH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9174 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.841, 67.552, 92.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.480 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23613 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | obtained by SeMet SAD phasing |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.498 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.880 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.208 | 1.862 |
| Number of reflections | 24666 | |
| <I/σ(I)> | 11 | 1.4 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 13.2 | 11.9 |
| CC(1/2) | 0.997 | 0.522 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 20% (w/v) PEG10,000 + 100 mM HEPES pH 7.5 |
