5MKK
Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 93.371, 93.371, 1043.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.900 - 2.700 |
| R-factor | 0.2284 |
| Rwork | 0.226 |
| R-free | 0.27150 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.110 | |
| Number of reflections | 77034 | |
| <I/σ(I)> | 16.3 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.7 | 11.7 |
| CC(1/2) | 0.997 | 0.276 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 26-30% PEG 400, 0.1 M Na3Citrate/HCl pH 3.5, 0.1 M Li2SO4, 0-2.5 mM CYMAL-5 |
