5MKB

Maltodextrin binding protein MalE1 from L. casei BL23 without ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91841
Spacegroup name	P 1
Unit cell lengths	41.036, 124.945, 143.739
Unit cell angles	74.51, 89.61, 89.86

Refinement procedure

Resolution	46.171 - 1.698
R-factor	0.1814
Rwork	0.178
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m28
RMSD bond length	0.003
RMSD bond angle	0.795
Data reduction software	XDS (VERSION November 3, 2014)
Data scaling software	XDS (VERSION November 3, 2014)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.171	1.760
High resolution limit [Å]	1.698	1.698
Rmerge	0.068	0.607
Number of reflections	294468
<I/σ(I)>	13.21	1.9
Completeness [%]	96.9	93.21
Redundancy	3.5	3.5
CC(1/2)	0.999	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 mM Na-Hepes, pH 7.0, 20-25% Jeffamine ED2003, pH 7.0