Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.7 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.082, 144.729, 96.767 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.840 - 2.160 |
| R-factor | 0.2223 |
| Rwork | 0.219 |
| R-free | 0.29690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lus |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.397 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.840 | 2.237 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.082 | 0.184 |
| Number of reflections | 12345 | |
| <I/σ(I)> | 19.63 | 7.06 |
| Completeness [%] | 92.6 | 99 |
| Redundancy | 5.6 | 5.6 |
| CC(1/2) | 0.996 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Tris-HCl 0.1M pH8.5 PEG 1500 26% Glycerol 16% |
