5MK2
Crystal structure of the His Domain Protein Tyrosine Phosphatase (HD-PTP/PTPN23) Bro1 domain (CHMP4B peptide complex structure)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.440, 64.860, 81.230 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 50.685 - 1.700 |
| R-factor | 0.1708 |
| Rwork | 0.169 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rau |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.748 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2563: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.685 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.041 |
| Number of reflections | 77355 |
| <I/σ(I)> | 18.56 |
| Completeness [%] | 98.7 |
| Redundancy | 3.3 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 0.2 M CaCl2, 0.1 M MES pH 6.0, 20% PEG 6K |
