5MJR
Structure of Psb29 at 1.55A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.660, 56.100, 44.550 |
| Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
| Resolution | 42.890 - 1.380 |
| R-factor | 0.11571 |
| Rwork | 0.114 |
| R-free | 0.15544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mjo |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.181 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.890 | 1.420 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.113 | 1.770 |
| Number of reflections | 37960 | |
| <I/σ(I)> | 5.1923 | 0.34 |
| Completeness [%] | 98.9 | 98.55 |
| Redundancy | 13.56 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.1 M trisodium citrate, 20% w/v PEG 6000 |
