5MJQ

Single-shot pink beam serial crystallography: Phycocyanin (One chip)

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	293
Detector technology	CCD
Collection date	2016-07-09
Detector	RAYONIX MX340-HS
Wavelength(s)	1.15-1.25
Spacegroup name	H 3 2
Unit cell lengths	187.800, 187.800, 60.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.917 - 2.700
R-factor	0.16
Rwork	0.154
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jbo
RMSD bond length	0.003
RMSD bond angle	0.769
Data reduction software	Epinorm
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.918	2.820
High resolution limit [Å]	2.700	2.700
Rmerge	0.041
Number of reflections	6647
<I/σ(I)>	46.56
Completeness [%]	59.1	0.269
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		293	Ammonium sulfate