5MJ7
Structure of the C. elegans nucleoside hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2005-10-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8075 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.578, 84.578, 260.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.930 - 1.650 |
| R-factor | 0.13807 |
| Rwork | 0.137 |
| R-free | 0.15405 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T. b. brucei IG-NH (pdb 3fz0) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.930 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.071 | 0.579 |
| Number of reflections | 110218 | |
| <I/σ(I)> | 44.9 | 2.3 |
| Completeness [%] | 96.2 | 76.2 |
| Redundancy | 22.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 300 | 20 mM Tris, pH 7.5, 150mM NaCl and 2mM DTT |
