5MJ2

Extracellular domain of human CD83 - rhombohedral crystal form after UV-RIP (S-SAD data)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.800042
Spacegroup name	H 3 2
Unit cell lengths	62.978, 62.978, 135.628
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.210 - 1.980
R-factor	0.1587
Rwork	0.156
R-free	0.20590
RMSD bond length	0.007
RMSD bond angle	1.067
Data reduction software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.210	2.100
High resolution limit [Å]	1.980	1.980
Rmerge	0.055	0.677
Number of reflections	14030
<I/σ(I)>	22.6	2.8
Completeness [%]	100.0	100
Redundancy	7.4	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 microliter protein (39 mg/ml in ultrapure water) and 0.4 microliter reservoir solution (0.2 M DL-malic acid (pH 7.0), 20% w/v PEG 3350) were mixed and equilibrated against 70 microliter of reservoir solution Crystals appeared after ~13 months