5MJ1

Extracellular domain of human CD83 - rhombohedral crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	H 3 2
Unit cell lengths	62.803, 62.803, 134.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.400 - 1.800
R-factor	0.1743
Rwork	0.172
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.137
Data reduction software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.400	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.053	0.740
Number of reflections	9772
<I/σ(I)>	21	2.2
Completeness [%]	99.8	99.7
Redundancy	5.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 microliter protein (39 mg/ml in ultrapure water) mixed with 0.4 microliter reservoir solution (0.2 M DL-malic acid (pH 7.0), 20% w/v PEG 3350) was equilibrated against 70 microliter of reservoir solution. Crystals appeared after ~ 13 months