5MJ0

Extracellular domain of human CD83 - cubic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918007
Spacegroup name	I 41 3 2
Unit cell lengths	149.564, 149.564, 149.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.296 - 3.200
R-factor	0.2388
Rwork	0.237
R-free	0.26360
RMSD bond length	0.006
RMSD bond angle	1.345
Data reduction software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	3.390
High resolution limit [Å]	3.200	3.200
Rmerge	0.078	1.353
Number of reflections	4984
<I/σ(I)>	25.9	2
Completeness [%]	99.7	99.2
Redundancy	12.6	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 microliter protein (21 mg/ml in 25 mM Tris-HCl (pH 7.2) buffer) were mixed with 0.2 microliter reservoir solution (0.2 M L-proline, 0.1 M HEPES (pH 7.5), 24% w/v PEG 1500) and equilibrated against 70 microliter of reservoir solution Crystals appeared after ~ 10 months