5MIY

Crystal structure of the E3 ubiquitin ligase RavN from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978870
Spacegroup name	C 2 2 2
Unit cell lengths	114.160, 177.010, 79.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.357 - 1.717
R-factor	0.1624
Rwork	0.161
R-free	0.19490
Structure solution method	SIRAS
Data reduction software	XSCALE
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.969	47.969	1.820
High resolution limit [Å]	1.720	5.120	1.720
Rmerge	0.055	0.032	0.874
Rmeas	0.057	0.033	0.910
Total number of observations	1134512
Number of reflections	85768	3443	13538
<I/σ(I)>	24.57	71.84	3.14
Completeness [%]	99.7	99.3	98.6
Redundancy	13.228	12.199	12.741
CC(1/2)	0.999	0.999	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	291	1.5 M Ammonium sulphate, 0.1 M NaCitrate pH 5.4 and 0.2 M K/Na Tartrate