5MIL
Pirating conserved phage mechanisms promotes promiscuous staphylococcal pathogenicity islands transfer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.470, 88.840, 54.150 |
| Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
| Resolution | 28.839 - 2.100 |
| R-factor | 0.1892 |
| Rwork | 0.186 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.138 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.130 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.127 | 0.308 |
| Number of reflections | 18124 | |
| <I/σ(I)> | 6.4 | 3.7 |
| Completeness [%] | 98.0 | 98.1 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2M magnesium chloride, 0.1M Tris pH8.5, 20% PEG 8000 |
