5MHS
T1L reovirus sigma1 complexed with 5C6 Fab fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.610, 129.080, 87.860 |
| Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
| Resolution | 48.991 - 3.700 |
| R-factor | 0.2618 |
| Rwork | 0.261 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4GU3 and 5MHR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.813 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.991 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmeas | 0.295 | 1.619 |
| Number of reflections | 26263 | |
| <I/σ(I)> | 7.94 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 11.957 | 12.4 |
| CC(1/2) | 0.991 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 9.5-11.5% (w/v) PEG8000, 0.1 M MES (pH 5.5-6.5), 0.2 M zinc acetate |
