5MHR
T3D reovirus sigma1 complexed with 9BG5 Fab fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 103.163, 109.306, 131.699 |
| Unit cell angles | 103.13, 113.56, 103.48 |
Refinement procedure
| Resolution | 49.050 - 3.000 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2OJ5 AND 1FIG |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.661 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.045 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 94667 | |
| <I/σ(I)> | 13.08 | 1.89 |
| Completeness [%] | 98.5 | 98.4 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 11% (w/v) PEG8000, 0.1 M Tris pH 7.0, 0.2 M magnesium chloride |
