5MHF
Murine endoplasmic reticulum alpha-glucosidase I with N-9'-methoxynonyl-1-deoxynojirimycin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.010, 130.920, 135.370 |
| Unit cell angles | 90.00, 99.53, 90.00 |
Refinement procedure
| Resolution | 133.500 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j5t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 133.500 | 2.107 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.133 | 1.164 |
| Number of reflections | 215063 | |
| <I/σ(I)> | 6.5 | 1 |
| Completeness [%] | 99.5 | 96.8 |
| Redundancy | 3.4 | 3 |
| CC(1/2) | 0.996 | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.09M Morpheus NPS, 0.1M Morpheus Buffer System 1 pH 6.5, 50% v/v Morpheus Precipitant Mix 1 |
