5MH3
Crystal structure of a DM9 domain containing protein from Crassostrea gigas with K43A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.930, 97.960, 101.420 |
| Unit cell angles | 101.34, 107.87, 106.46 |
Refinement procedure
| Resolution | 19.987 - 1.600 |
| R-factor | 0.1702 |
| Rwork | 0.169 |
| R-free | 0.19640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DM9CP |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.052 | 1.070 |
| Number of reflections | 261115 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 95.5 | 89 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M Bis-Tris pH 6.0 25%PEG 3350 0.2M Lithium sulphate monohydrate |
