5MH1

Crystal structure of a DM9 domain containing protein from Crassostrea gigas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.999999
Spacegroup name	P 32 2 1
Unit cell lengths	67.300, 67.300, 55.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 1.100
R-factor	0.1008
Rwork	0.100
R-free	0.11690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mh0
RMSD bond length	0.008
RMSD bond angle	1.137
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.200
High resolution limit [Å]	1.100	1.100
Rmeas	0.072	2.000
Number of reflections	58075
<I/σ(I)>	21.7	1.4
Completeness [%]	99.9	95.9
Redundancy	17	14.5
CC(1/2)	0.990	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.1M MES, pH 6.0, 20% PEG 6000, 10 mM mannose