5MGT

Complex of human NKR-P1 and LLT1 in deglycosylated forms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	44.580, 80.150, 272.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.910 - 1.900
R-factor	0.162
Rwork	0.162
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bpd
RMSD bond length	0.019
RMSD bond angle	1.811
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.910	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.153	0.976
Number of reflections	78617
<I/σ(I)>	14	3
Completeness [%]	100.0	99.9
Redundancy	18.5	18
CC(1/2)	0.998	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	0.200 M Ammonium Sulphate, 20 %w/v Polyethylene Glycol MME 5000, 0.1 M Tris pH 7.5, total protein concentration 8 mg/ml