5MGR
Human receptor NKR-P1 in glycosylated form, extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.241, 68.241, 127.195 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.300 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ff7 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.736 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.637 |
| Number of reflections | 32555 | |
| <I/σ(I)> | 41 | 7.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 39.5 | 40.5 |
| CC(1/2) | 1.000 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 294 | 20% (w/v) PEG 3350, 200 mM di-sodium tartrate pH 7.2 PROTEIN CONCENTRATION 20 MG/ML |
