5MGA
Structure of the Cpf1 endonuclease R-loop complex after DNA cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.223, 137.652, 320.513 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.580 - 3.000 |
| R-factor | 0.2428 |
| Rwork | 0.242 |
| R-free | 0.26530 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.649 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.120 | 2.960 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 1.750 | |
| Number of reflections | 39708 | |
| <I/σ(I)> | 7 | 1.3 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.35M sodium thiocyanate and 20.9% w/v PEG 3350 |
