5MFO
Designed armadillo repeat protein YIIIM3AIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 |
| Unit cell lengths | 83.920, 83.920, 166.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.287 - 1.300 |
| R-factor | 0.1596 |
| Rwork | 0.159 |
| R-free | 0.17810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.898 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.287 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.056 | 1.695 |
| Number of reflections | 278605 | |
| <I/σ(I)> | 19.48 | 1.44 |
| Completeness [%] | 99.3 | 98.1 |
| Redundancy | 11.2 | 11.1 |
| CC(1/2) | 1.000 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.2 M Ca-Acetate, 0.1 M Na-Acetate pH 5.5, 25 % PEG 2K MME |
