5MFN
Designed armadillo repeat protein YIIIM5AII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 142.550, 142.550, 142.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.660 - 2.800 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mfc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.257 | 7.643 |
| Number of reflections | 21564 | |
| <I/σ(I)> | 18.68 | 0.69 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 39.7 | 37.4 |
| CC(1/2) | 1.000 | 0.184 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Sodium malonate 2.4M pH 7.0, 0.2M CaCl2 |
