5MFF
Designed armadillo repeat protein YIIIM5AII in complex with peptide (RR)5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.755, 90.886, 179.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.310 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.310 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 4.680 |
| Number of reflections | 106118 | |
| <I/σ(I)> | 14.51 | 0.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11 | 11.2 |
| CC(1/2) | 0.999 | 0.270 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 60% (NH4)2 SO4, 0.1M HEPES pH 8.0 |
