5MEK
Sulphotransferase-18 from Arabidopsis thaliana in complex with 3'-phosphoadenosine 5'-phosphate (PAP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.740, 62.740, 201.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.740 |
| R-factor | 0.16946 |
| Rwork | 0.167 |
| R-free | 0.20755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB-ID: 1Q44 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.850 | 1.840 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.093 | 0.753 |
| Number of reflections | 42500 | |
| <I/σ(I)> | 20.44 | 2.65 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.66 | 22.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.9 | 291.15 | 0.1 M MES pH 5.9, 16.5% PEG4000, 160 mM NaCl and 5% 1-propanol |
