5MEC

Crystal structure of yeast Cdt1 middle domain (residues 294-433)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	45.130, 46.330, 75.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.468 - 2.130
R-factor	0.1989
Rwork	0.197
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2WVR chain C residues 188-315 truncated to polyALA
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.470	2.190
High resolution limit [Å]	2.130	2.130
Rmerge	0.078	0.620
Number of reflections	9296
<I/σ(I)>	15	3.3
Completeness [%]	99.5	99.2
Redundancy	6.9	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M BTP pH 5.5, 2M ammonium sulphate