5MEB
Crystal structure of yeast Cdt1 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97246 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.930, 85.870, 89.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.595 - 1.800 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.21000 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (phenix.autosolve) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 1.910 |
| Number of reflections | 31908 | |
| <I/σ(I)> | 20.2 | 1.91 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 18 | 18.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% glycerol, 0.1M MES pH 6.5, 1.8M ammonium sulphate |
