5ME9
Crystal structure of yeast Cdt1 (N terminal and middle domain), form 1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.897, 122.545, 148.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.197 - 2.700 |
| R-factor | 0.2006 |
| Rwork | 0.199 |
| R-free | 0.23110 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.767 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (phenix.autosolve) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.197 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.120 | 1.400 |
| Number of reflections | 42303 | |
| <I/σ(I)> | 15.8 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% Glycerol, 20% PEG 4000, 20% 2-Propanol, 0.1M Tris-HCl pH8.5 |
