5ME9

Crystal structure of yeast Cdt1 (N terminal and middle domain), form 1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	82.897, 122.545, 148.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.197 - 2.700
R-factor	0.2006
Rwork	0.199
R-free	0.23110
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.767
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (phenix.autosolve)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.197	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.120	1.400
Number of reflections	42303
<I/σ(I)>	15.8	2.2
Completeness [%]	100.0	100
Redundancy	11.6	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	30% Glycerol, 20% PEG 4000, 20% 2-Propanol, 0.1M Tris-HCl pH8.5