5ME7

Crystal Structure of eiF4E from C. melo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	57.746, 108.957, 122.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.1881
Rwork	0.187
R-free	0.21392
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.951
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.310	47.080	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge		0.087	0.384
Number of reflections	39619
<I/σ(I)>	7.9	5.8	2
Completeness [%]	99.3	98.7	97.8
Redundancy	3.7	3.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	sodium potassium tartrate