5MDU
Structure of the RNA recognition motif (RRM) of Seb1 from S. pombe.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.130, 47.170, 32.360 |
| Unit cell angles | 90.00, 98.88, 90.00 |
Refinement procedure
| Resolution | 43.340 - 1.020 |
| Rwork | 0.135 |
| R-free | 0.15180 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | xia2 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 1.050 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.045 | 0.748 |
| Number of reflections | 79454 | |
| <I/σ(I)> | 15.5 | 1.3 |
| Completeness [%] | 94.6 | 57.5 |
| Redundancy | 6 | 3.2 |
| CC(1/2) | 0.999 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.65 | 1 M ammonium formate, 100 mM sodium cacodylate, 8% (w/v) poly-gamma-glutamic acid polymer (PGA-LM, 200-400 kDa low molecular weight polymer) |
