5MDM
Structural intermediates in the fusion associated transition of vesiculovirus glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 150.340, 228.210, 78.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.108 - 2.998 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2CMZ |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 2.998 | 2.998 |
| Rmerge | 0.090 | 1.253 |
| Number of reflections | 55165 | |
| <I/σ(I)> | 16.7 | 1.5 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 14% PEG 3350, 0.3 M Na2SO4 and 0.1 M HEPES pH 7.6; final pH 7.5 |
