5MC7
Crystal structure of Truncated Human Coatomer Protein Complex, subunit Z1 (CopZ1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.360, 65.403, 37.273 |
| Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
| Resolution | 56.030 - 1.600 |
| R-factor | 0.21243 |
| Rwork | 0.210 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hf6 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.569 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.030 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.046 | 0.370 |
| Number of reflections | 33495 | |
| <I/σ(I)> | 16 | 2.6 |
| Completeness [%] | 97.3 | 95.4 |
| Redundancy | 3.0246 | 3 |
| CC(1/2) | 0.998 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 2.2 M DL-Malic acid 0.2 M Na Malonate |
