5MC7

Crystal structure of Truncated Human Coatomer Protein Complex, subunit Z1 (CopZ1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033200
Spacegroup name	C 1 2 1
Unit cell lengths	109.360, 65.403, 37.273
Unit cell angles	90.00, 96.58, 90.00

Refinement procedure

Resolution	56.030 - 1.600
R-factor	0.21243
Rwork	0.210
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hf6
RMSD bond length	0.030
RMSD bond angle	2.569
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.030	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.046	0.370
Number of reflections	33495
<I/σ(I)>	16	2.6
Completeness [%]	97.3	95.4
Redundancy	3.0246	3
CC(1/2)	0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	295	2.2 M DL-Malic acid 0.2 M Na Malonate