5MC0
Crystal Structure of delTyr231 mutant of Human Prolidase with Mn ions and GlyPro ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.943, 108.113, 211.927 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.260 - 1.560 |
R-factor | 0.118 |
Rwork | 0.116 |
R-free | 0.15300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m4j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.213 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.153 | 1.650 |
High resolution limit [Å] | 1.558 | 1.560 |
Rmerge | 0.088 | 0.979 |
Number of reflections | 168926 | |
<I/σ(I)> | 14.04 | 1.77 |
Completeness [%] | 99.7 | 98.4 |
Redundancy | 7.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 278 | 10mM NaBorate, 640mM NaCitrate |