5MBS
Crystal structure of Bacillus subtilis EngA in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.920, 64.150, 62.010 |
| Unit cell angles | 90.00, 96.39, 90.00 |
Refinement procedure
| Resolution | 61.620 - 3.200 |
| R-factor | 0.2277 |
| Rwork | 0.220 |
| R-free | 0.37252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dcu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.337 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.620 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.744 | |
| Number of reflections | 6572 | |
| <I/σ(I)> | 9.69 | 1.67 |
| Completeness [%] | 98.5 | 96.5 |
| Redundancy | 3.27 | 3.21 |
| CC(1/2) | 0.996 | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.5 M sodium sulfate 0.1 M Tris pH 6.5 16% PEG 3350 |
