5MAW

Crystal structure of the flagellin-FliS complex from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9723
Spacegroup name	P 1 21 1
Unit cell lengths	52.138, 62.353, 65.078
Unit cell angles	90.00, 111.33, 90.00

Refinement procedure

Resolution	48.568 - 1.500
R-factor	0.174
Rwork	0.173
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1io1 1vh6
RMSD bond length	0.010
RMSD bond angle	0.945
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.570	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.044	0.230
Number of reflections	61470	6152
<I/σ(I)>	18.17	5.4
Completeness [%]	99.0	99
Redundancy	4.1	4.2
CC(1/2)	0.998	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	0.2 M Lithium sulfate, 0.1 M Tris pH 7.0, 2.0 M Ammonium sulfate