5MAV
Crystal structure of human PCNA in complex with PARG (poly(ADP-ribose) glycohydrolase) peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.968 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.597, 153.604, 85.512 |
Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
Resolution | 47.671 - 2.575 |
R-factor | 0.2412 |
Rwork | 0.239 |
R-free | 0.29090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vym |
RMSD bond length | 0.007 |
RMSD bond angle | 1.041 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.671 |
High resolution limit [Å] | 2.570 |
Rmerge | 0.260 |
Rmeas | 0.293 |
Rpim | 0.132 |
Number of reflections | 62473 |
<I/σ(I)> | 5.6 |
Completeness [%] | 99.1 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1 M Tris-HCl pH 7.5, 20% PEG 3350, 0.2 M ammonium acetate |