5MAO

HERA HELICASE RNA BINDING DOMAIN with TNCS in P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-06-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.999900
Spacegroup name	P 21 21 21
Unit cell lengths	38.510, 48.270, 64.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.747 - 1.350
R-factor	0.2385
Rwork	0.238
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i31
RMSD bond length	0.005
RMSD bond angle	0.802
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11_2558)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.750	38.750	1.390
High resolution limit [Å]	1.350	6.040	1.350
Rmerge	0.124	0.045	1.690
Rmeas	0.139
Total number of observations	129298
Number of reflections	26573
<I/σ(I)>	6.67	19.59	0.86
Completeness [%]	97.1	97	96
Redundancy	4.87
CC(1/2)	0.997	0.997	0.286

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	unknown