5MAL

Crystal structure of extracelular lipase from Streptomyces rimosus at 1.7A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.999
Spacegroup name	P 1 21 1
Unit cell lengths	38.090, 78.690, 56.560
Unit cell angles	90.00, 104.49, 90.00

Refinement procedure

Resolution	24.007 - 1.708
R-factor	0.1676
Rwork	0.165
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hyq
RMSD bond length	0.007
RMSD bond angle	1.237
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.007	24.007	1.800
High resolution limit [Å]	1.708	5.400	1.710
Rmerge		0.059	0.326
Rmeas	0.108
Rpim	0.055
Total number of observations	131164
Number of reflections	34875
<I/σ(I)>	9.3	7.1	2.1
Completeness [%]	99.5	98.8	96.5
Redundancy	3.8	3.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M MES, 25 % PEG 2000 MME