5MAK
GFP-binding DARPin fusion gc_R7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.960, 61.380, 89.380 |
| Unit cell angles | 93.12, 102.74, 94.76 |
Refinement procedure
| Resolution | 43.525 - 2.500 |
| R-factor | 0.2573 |
| Rwork | 0.255 |
| R-free | 0.30360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.506 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.525 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.141 | 0.860 |
| Number of reflections | 39327 | |
| <I/σ(I)> | 8.62 | 1.86 |
| Completeness [%] | 95.1 | 97.2 |
| Redundancy | 3.5 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Tri Sodium Citrate pH 5.6, 20% v/v 2-Propanol, 20%w/v PEG 4000 |
