5MA6
GFP-binding DARPin 3G124nc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 70.310, 70.310, 432.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.300 |
| R-factor | 0.20684 |
| Rwork | 0.205 |
| R-free | 0.24096 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.559 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.148 | 8.520 |
| Number of reflections | 29482 | |
| <I/σ(I)> | 17.55 | 0.68 |
| Completeness [%] | 98.7 | 98.5 |
| Redundancy | 37.3 | 40.7 |
| CC(1/2) | 1.000 | 0.416 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Na-Acetate pH 5.5, 0.5M KH2PO4 |
