5MA4
GFP-binding DARPin fusion gc_K7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.880, 92.340, 56.530 |
| Unit cell angles | 90.00, 114.60, 90.00 |
Refinement procedure
| Resolution | 47.293 - 1.400 |
| R-factor | 0.1432 |
| Rwork | 0.142 |
| R-free | 0.17040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.275 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.293 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.053 | 0.892 |
| Number of reflections | 102263 | |
| <I/σ(I)> | 17.63 | 2.11 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.4 | 5.2 |
| CC(1/2) | 0.999 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Sodium Acetate trihydrate, 0.1M TRIS HCl pH 8.5, 30%w/v PEG 4000 |
