5M9S
Human angiogenin ALS variant V103I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.749, 115.906, 37.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.350 - 1.850 |
| R-factor | 0.21218 |
| Rwork | 0.208 |
| R-free | 0.26447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.492 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.400 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 14125 | |
| <I/σ(I)> | 6.3 | 1 |
| Completeness [%] | 90.7 | 76.3 |
| Redundancy | 6.5 | 2.8 |
| CC(1/2) | 0.993 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2 M Na/K tartrate 0.1 M MES 20% PEG 4000 |
