5M9R
Human angiogenin ALS variant F100I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.878, 34.781, 52.920 |
| Unit cell angles | 89.72, 84.17, 84.34 |
Refinement procedure
| Resolution | 52.650 - 1.440 |
| R-factor | 0.17341 |
| Rwork | 0.172 |
| R-free | 0.19544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.621 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 1.470 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Number of reflections | 37557 | |
| <I/σ(I)> | 6.5 | 3.9 |
| Completeness [%] | 95.1 | 80.1 |
| Redundancy | 2.4 | 2.3 |
| CC(1/2) | 0.989 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2 M Na/K tartrate 0.1 M Na cacodylate 20% PEG 4000 |
