5M9J
Human angiogenin PD variant K60E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9700 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.046, 117.072, 37.275 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.500 - 1.900 |
| R-factor | 0.18172 |
| Rwork | 0.178 |
| R-free | 0.21551 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ang |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.500 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 14137 | |
| <I/σ(I)> | 14.2 | 2.6 |
| Completeness [%] | 96.2 | 71.2 |
| Redundancy | 4.8 | 4.5 |
| CC(1/2) | 0.992 | 0.626 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2 M Na/K tartrate 0.1 M Na cacodylate 25% PEG 4000 |
